XX University, Depatment of XX
Dear Dr. XXX,
I would like to be considered for the advertised (Science, Vol. XX) Assistant Professor position in your department. My research interest is to elucidate the signaling mechanisms underlying synaptic plasticity, and ultimately learning and memory, using novel quantitative and biophysical methods.
As part of my graduate work with Dr. Kazahara Kanasata (Kemeo University, Yomomama, Japan) I have shown for the first time that the enzyme F1-ATPase is a rotary motor. I measured the properties of individual motors using optical techniques and revealed that F1-ATPase is a three-stepped motor operating at close to 100% energy efficiency. In recognition of these measurements I received the Young Fluorescence Investigator Award from the Fluorescence Subgroup of the Biophysical Society.
After a short postdoc in my thesis lab, I moved to Cold Spring Harbor Laboratory to learn neuroscience. Under the mentorship of Dr. Karal Svabada, I began by working on the regulation of voltage-gated calcium channels in neurons. Using two-photon calcium imaging with single channel sensitivity, in combination with electrophysiology, I found that calcium channels are regulated by Ca2+ for long times, and that this regulation modulates synaptic plasticity.
For a deeper understanding of signal transduction, techniques to directly visualize enzymatic activities and protein-protein interactions are required. I set out to develop two-photon fluorescence lifetime imaging microscopy with which fluorescence resonance energy transfer can be measured quantitatively. In support of this research program I received a Career Award at the Scientific Interface from the Burroughs Wellcome Fund.
Using my toolbox of quantitative biophysical techniques, my long term objective is to decipher the signaling mechanisms underlying various brain functions and dysfunction. I am confident that I am ready to run an innovative independent research laboratory.
Enclosed please find my curriculum vitae and a brief description of my research plans. Please feel free to contact me if you should need additional information.
Ryohei Yasuda, Ph.D.
Cold Spring Harbor Lab.,
1 Bungtown Road, Cold Spring Harbor, NY11724